Webshop

Order all your oligos quick and easy in our webshop. With your own account you can directly see the prices of oligos. Have direct access to your order status, tracking information and order history. All this is packaged in an easy-to-use on-line interface.

Email

Download our excel order form and send the completed form to info@biolegio.com.

See here our webshop video guide 

Modifications can be incorporated either during (direct) or after (indirect) synthesis. You can choose from over 300 modifications. If your desired modification is not listed on our website, please contact us.     

View modifications

How can I add a modification to my oligo?
Select the modifications of your choice in our webshop or in our excel ordering sheet. In the webshop you can have instant insight in pricing. 

How can I add mixed bases to my oligo?

Use IUB Codes for mixed base sites:

K = G, T
R = G, A
W = A, T
B = G, T, C
H = A, T, C
N = G, A, T, C
M = A, C
S = G, C
Y = T, C
D = G, A, T
V = G, A, C

How can I order DNA/RNA hybrid oligos?
DNA/RNA hybrid oligos can be ordered in with some minor adjustments in the sequence. Mark the RNA bases by placing a lower case ‘r’ in front of them. For DNA bases place a lower case ‘d’ in front. For ‘chemistry’ choose DNA/RNA hybrid.

All DNA oligos are purified by a propriety desalting method. This avoids the need for purification for most basic molecular biology applications. Additional purification is available for more sensitive applications. 

We offer three different forms of supplemental purification:

• Reverse-Phase cartridge purification
• HPLC
• PAGE

More about purification

Oligo scale and length
Biolegio offers four different synthesis scales for DNA oligos: 10 nmol, 40 nmol, 200 nmol and 1000 nmol. For each synthesis scale there is a restriction regarding the length of the oligo.

Guaranteed yield standard oligos
For non-labeled, non-HPLC/PAGE purified standard DNA oligos up to 30 bases we guarantee a minimum yield. However in many cases you will receive more than the guaranteed minimum!

Method
Biolegio has two synthesis protocols:  ‘High Purity’ and ‘B-pure’. The used protocol depends on the length of the oligo. This means that the protocols have been optimized for certain oligo lengths.

• High purity protocol is used for oligos between 1 and  79 bases.
• All DNA oligos longer than 80 bases are synthesized on our B-pure synthesis protocol.

Each protocol delivers the highest possible coupling efficiency for the specified range.