Custom DNA oligos can be ordered in our webshop by entering your sequences and specifications (up to 200 bases) or by email. Login to our webshop and order your oligo's

For medium and high-throughput applications oligos can be ordered in plates. No additional charge for orders containing more than 48 oligos per plate. More information about DNA synthesis in plates.

Order your primes and/or probes delivered in any format according to your specifications. All fluorophore -quencher combinations possible, ideal for qPCR applications.

Oligonucleotides up to and beyond 200 bases are achieved with our B-pure Oligonucleotide Synthesis protocol. This  protocol enables high quality long oligo synthesis at an affordable price. By using the B-pure protocol we can achieve a coupling efficiency >99,5%. 

Oligonucleotides longer than 80 bases are synthesized automatically on this protocol. Additional quality control is performed on our state-of-the-art LC-MS system to guarantee highest quality and optimized functionality of the oligonucleotides.

With our oligo prepaid service we make oligo ordering easy. Credit can be easily transferred to your account. Oligos can then be ordered immediately, eliminating possible delays due to purchase authorization. Please contact Biolegio for more information.

Your DNA oligo can be shipped dry or dissolved, annealed or mixed. Standard DNA oligos are delivered dissolved in a tube in a concentration of 100 µM (100 pmol/μl). Oligo's ordered dissolved on the 1000nmol synthesis scale will be delivered in a concentration of 1mM (1 nmol/µl).

Available buffers

• H2O buffer

         Demineralized water with a resistance of ...Ohm

• LoTE buffer

          TE buffer with a reduced concentration of EDTA

• TE buffer

           The standard at Biolegio with a pH value of 8.0 for optimal stability

DNA oligos can be delivered in any format (plates, tubes, etc.), concentration or combination. Have a look at our oligo dispensing service for more information about this topic.

All DNA oligos are synthesized under low salt conditions. This avoids the need for purification for most basic molecular biology applications. Additional purification is available for more sensitive applications. Furthermore, we recommend purification for oligos longer than 40 bases. 

We offer three different forms of purification:

• Standard desalting
• HPLC
• PAGE

Modifications can be incorporated either during (direct) or after (indirect) synthesis. You can choose from over 300 modifications. If your desired modification is not listed on our website, please contact us.

How can I add a modification to my oligo?

Select the modifications of your choice in our webshop or in our excel ordering sheet. In the webshop you can have instant insight in pricing. 

How can I add mixed bases to my oligo?

Use the standard code letters for mixed bases / wobbles. 

How can I order DNA/RNA hybrid oligos?

DNA/RNA hybrid oligos can be ordered in with some minor adjustments in the sequence. Mark the RNA bases by placing a lower case 'r' in front of them. For DNA bases place a lower case 'd' in front. For 'chemistry' choose DNA/RNA hybrid.

Unmodified oligos can be used for at least 12 months when stored at -20°C. Use nuclease-free, sterile water for working solutions. Oligonucleotides modified with fluorescent dyes should be kept away from light as much as possible. Avoid repeated freeze-thaw cycles as this process can lead to degradation of the oligo.

All your DNA oligos are delivered with a specification sheet including GC-content, molecular weight, melting temperature (Tm), OD value, sequence and quantity in μgram and nmol. 

A stringent quality control system ensures that you can expect the quality of our oligonucleotides to be of the highest standard. Before the synthesis, all chemicals are checked to meet our quality standards. During the fully automated oligo synthesis, all steps of the synthesis are monitored by multiple control functions on our DNA synthesizers. This way we can assure the coupling efficiency of the synthesis to meet our demands.

Synthesis is followed by further quality controls to guarantee the quality of the oligos. All oligos are routinely analyzed by optical density (OD) measurement. In addition, oligos are analyzed by gel electrophoresis and Liquid Chromatography Mass Spectrometry (LCMS). If oligos do not meet our requirement they are resynthesized. The result of our stringent QC process is a product of the highest standard. All oligos are delivered with our product specification sheet, which includes: % GC content, yield in nmol and μgram, OD, melting temperature and molecular weight (MW).