Advanced RNA

 

Synthego - Advanced RNA

Highest quality synthetic RNA with the flexibility and quality to achieve your goals. Up to 103nts in length.

Complete Flexibility

Flexibility in RNA length

Advanced RNA 50 - 20-50 nt Sequences

Advanced RNA 75 - 51-75 nt Sequences

Advanced RNA 100 - 76 -103 nt Sequences

Synthesise guide RNAs that are compatible with any CRISPR nuclease

Including all engineered versions of SpCas9, Cas12a (Cpf1), SaCas9 and Cas13a.

Also alter the secondary structure of the guide RNAs to your requirements

Scalable Synthesis
High Purity Synthetic RNA
Fast turnaround time
 
01

Advanced RNA 50

Custom RNA 50 is perfect for designing short guide RNAs for nucleases that don’t require long sequences, such as Cpf1. You can use it to design your own Cas9 crRNA and custom 3′ linker to a tracrRNA.

 
02

Advanced RNA 75

Custom RNA 75 is perfect for designing guide RNAs for nucleases that don’t require guides as long as S. pyogenes Cas9. You can use it to design your own Cas9 tracrRNA and custom 5′ linker to a crRNA.

 
03

Advanced RNA 100

Custom RNA 100 is perfect for designing full length sgRNA for CRISPR at a practical scale and price point. You can custom design your entire sgRNA sequence from 76-103 nucleotides.

 
04

Custom RNA Specifications

  • Custom RNA Specifications
  • Product Type: Fully Synthetic RNA for CRISPR or other applications
  • Purification: High-Quality Liquid Chromatography SPE
  • Analysis Method: Electrospray Ionization Mass Spectrometry
  • Shipping Format: Nuclease-free Tubes
 
05

Stem cells

Working with stem cells or other difficult targets? Try Chemically Modified Synthetic RNA.

Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit. For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. 

 

Advanced RNA 100

 

  • Your 76-103nt Sequence
  • Material for 10-20 Transfections
  • Quantity: 3 nmol by OD260
  • Modified RNA Optional*
  • Ships in 5-10 Business Days
     

 

Advanced RNA 75

 

  • Your 51-75nt Sequence
  • Material for 25-50 Transfections
  • Quantity: 5 nmol by OD260
  • Modified RNA Optional*
  • Ships in 5-10 Business Days 

 

 

Advanced RNA 50

 

  • Your 20-50nt Sequence
  • Material for 25-50 Transfections
  • Quantity: 5 nmol by OD260
  • Modified RNA Optional*
  • Ships in 5-10 Business Days