The LegiSeq™ Universal Short Adapter is a truncated adapter for Illumina® NGS. The adapters are TA-ligated to library fragments following an A-tailing step and are used with the LegiSeq™ UDI Primers to incorporate Unique (10 nucleotide) Indexes via a subsequent PCR step.

•   Produced under optimized NGS Production and Handling Workflows
  • Minimising Cross Contamination & Index Hopping risk
  • Highest quality Oligos -optimized for NGS
  • High ligation efficiency, low adapter-dimer formation
• NGS Quality Control check performed on every production batch
  • Ensuring Cross Contamination levels <0.01%
• Compatible with 2 & 4 Colour Illumina® Sequencing Platforms
• Indexes are designed with an edit distance ≥3 (Hamming distance)
• Cross referenced for no duplicate indexes across different Biolegio Index sets and no conflict against index sets from other main suppliers and library preparation kit manufacturers
• All manufactured under a ISO9001:2015 & ISO13485:2016 Accredited Quality Management System

Available in Sets of 16, 96 and 384

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LegiSeq™ Universal Short Adapter & UDI Indexing Primer Sets

Universal Short Adapter and unique dual indexing primers provided together- For Illumina® sequencing TA-ligation based library preparation (TruSeq™ Compatible). Available as ready-to-use, single-use plates of 16 or 96 UDI primer pairs (10nt Indexes) and an accompanying tube of Universal Short Adapters with a corresponding number of reactions.

Catalogue Number                        Description       

647001                                                LegiSeq™ Universal Short Adapter & UDI Primers, 16rxns

647002                                                LegiSeq™ Universal Short Adapter & UDI Primers, 96 rxns- Plate 1

647026                                                LegiSeq™ Universal Short Adapter & UDI Primers, 96 rxns- Plate 2

647027                                                LegiSeq™ Universal Short Adapter & UDI Primers, 96 rxns- Plate 3

647028                                                LegiSeq™ Universal Short Adapter & UDI Primers, 96 rxns- Plate 4

647040                                                LegiSeq™ Universal Short Adapter & UDI Primers, 384 rxns- Plates 1-4

LegiSeq™ Universal Short Adapter

Universal Short Adapter only- For Illumina® sequencing TA-ligation based library preparation. Sample Indexing/Barcoding performed via PCR using Biolegio Indexing Primers (sold separately).

Catalogue Number                        Description       

647003                                                LegiSeq™ Universal Short Adapter, 16rxns

647004                                                LegiSeq™ Universal Short Adapter, 96 rxns

647041                                                LegiSeq™ Universal Short Adapter, 384 rxns

LegiSeq™ UDI Indexing Primers 

Premixed Primer Pairs, for use with LegiSeq™ Universal Short Adapters (sold separately) for Illumina® sequencing TA-ligation based library preparation. Available as ready-to-use, single-use plates of 16, 96 or 384 primer pairs. 10 nt UDI indexes. Bulk volumes also available for high throughput requirements, contact info@biolegio.com for further information.

Catalogue Number                        Description   

647005                                                LegiSeq™ UDI Primers, 16 rxns

647006                                                LegiSeq™ UDI Primers, 96 rxns- Plate 1

647007                                                LegiSeq™ UDI Primers, 96 rxns- Plate 2

647008                                                LegiSeq™ UDI Primers, 96 rxns- Plate 3

647009                                                LegiSeq™ UDI Primers, 96 rxns- Plate 4

647010                                                LegiSeq™ UDI Primers, 384 rxns- Plates 1-4