Figure 1. - Our Cross contamination QC- 96 Unique Dual Indexes are attached to samples containing unique Index Spike-In sequences in each well. The subsequent unique combination of sequencing barcodes and spike-in sequence are defined in known "co-ordinates" in the final library. Following Sequencing during data analysis a 96 x 96 array is generated to confirm the presence of the barcode and spike-in the expected position, with a QC clearance criteria that the sequencing barcodes are detected in <0.1% of reads in any other position.  

Available as an optional QC check on Custom NGS Oligos and as a standard QC on every production batch of Biolegio Ready-to-Use, pre-configured Adapter and Indexing Primer sets our dedicated NGS based quality control check was developed In-House and based upon the Sample Assurance Spike-In Sequencing (SASI-Seq) assay method.

This check confirms cross-contamination levels of co-synthesised Index sequences within a given the set are well below industry standard for equivalent NGS Oligos.

We typical observe cross contamination values in the region of 0.05% and observe levels as low as 0.01% across full sets of 16 & 96 Adapters and Index primers with a minimal QC clearance and release value <0.1% for every production batch.

Additional functional QC demonstrate high ligation efficiency, consistent library yields and clustering efficiency and that our adapters and index primers meet the needs of your multiplexed sequencing applications.