Indexes and Adapters for NGS

NGS Adaptors and Indexes

 

NGS oligonucleotides often contain specific Adaptor sequences for library fragment recognition by the NGS platform. Another key aspects in NGS is Sample Multiplexing which is enabled by Indexing strategies. With the throughput of sequencing platforms increasing, there have been several established indexing strategies to enable high levels of multiplexing.

 

Biolegio optimized Adaptor and Index use in collaboration with multiple customers. The high-quality oligo synthesis with mitigated cross-contamination performed at Biolegio will help you to achieve sequencing results you can rely on!

Mitigate cross-contamination

At Biolegio we offer two NGS oligonucleotide grades: NGS-Standard and NGS-Premium. 

For both categories we give the same attention to the highest quality production workflow to achieve cross-contamination levels below 0.1%

NGS Standard

Highest synthesis quality

 

Maximal reduced cross contamination

NGS Premium

HPLC purified oligonucleotides

 

Full LC-MS report

 

Highest synthesis quality

Synthesis quality

Biolegio NGS oligonucleotides are synthesized in an environment which is monitored by externally executed qPCR assays using swaps of the facility, hardware and technicians.